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Also, the threshold degree of CYC9 or CRK12 essential for every of those features can be diverse, to make sure that depletion of CYC9 or CRK12 might be sufficient to disrupt cytokinesis or endocytosis, although not both.To research the subcellular localization with the CRK12 protein, a pEarleyGate104 vector was useful for a transient expression of